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In a previous post we commiserated with drug discovery scientists and their IT colleagues in their daily struggles to deal with the ever-increasing research data deluge. We then attempted to ease their ...
Data management, image processing, multivariate statistical analysis, and profiling at both individual cell and aggregated well level are increasingly becoming a bottleneck in HCS analysis. In this Webinar we will present an autophagy assay across three cancer cell lines to validate and automate a phenotypic HCS analysis workflow by PerkinElmer’s Columbus and Spotfire High Content Profiler. We will start with our instrument-agnostic HCS platform Columbus which stores, manages and analyzes images coming from all common HCS instruments. Even complex analysis sequences can be easily created by using our famous “Building Block” approach. The results of that image analysis can be selected directly within Spotfire High Content Profiler (HCP), our solution for the analysis and visualization of multiparametric HCS data. Spotfire HCP creates different graphics and analysis in an automated workflow starting with QC overviews on single cell and well-level data but also statistical analysis like Principle Component Analysis and unsupervised machine learning algorithm. All these tools help the end-user to validate their data and find interesting Compounds, RNAis or new classes in their data.
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